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	Provedor de dados Genet. Mol. Biol. (3) BABT (1) Autor Zaha,Arnaldo (4) Vasconcelos,Ana Tereza Ribeiro de (3) Alarcón,Frank (1) Almeida,Darcy Fontoura de (1) Becker,Daniela (1) Brocchi,Marcelo (1) Cortez-Herrera,Elizabeth (1) Kritski,Afrânio (1) Morais,David Anderson de Lima (1) Rossetti,Maria Lucia Rosa (1) Souza,Rangel Celso (1) Sperhacke,Rosa Dea (1) Yim,Lucia (1) Mais... Palavra-chave Genomes (2) Mycoplasma spp (2) Bidirectional best hit (1) Cell division (1) Comparative genomics (1) Internal control (1) Mollicutes (1) Nucleic acid amplification (1) Polymerase Chain Reaction (1) Restriction-modification systems (1) Synteny (1) Tuberculosis (1) Uracyl DNA glycosilase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2008 (1) 2007 (3) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Genes involved in cell division in mycoplasmas Genet. Mol. Biol. Alarcón,Frank; Vasconcelos,Ana Tereza Ribeiro de; Yim,Lucia; Zaha,Arnaldo. Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes involved in cell division processes. The division and cell wall (dcw) cluster, which in E. coli and B. subtilis is composed of 16 and 17 genes, respectively, is represented by only three to four genes in mycoplasmas. Even the most conserved protein, FtsZ, is not present in all mycoplasma genomes analyzed so far. A model for the FtsZ protein from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell division; Mycoplasma spp; Genomes. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200003 In search of essentiality: Mollicute-specific genes shared by twelve genomes Genet. Mol. Biol. Souza,Rangel Celso; Almeida,Darcy Fontoura de; Zaha,Arnaldo; Morais,David Anderson de Lima; Vasconcelos,Ana Tereza Ribeiro de. Mollicutes are cell wall-less bacteria with a genome characterized by its small size. Chromosomal rearrangements help these organisms evade host immune surveillance and hence cause disease. Our goal was to determine genes shared by Mollicutes genomes using the bidirectional best hit methodology. The twelve studied Mollicutes share 210 genes, most of which (> 60%) fall into the following COG categories: translation, ribosomal structure and biogenesis; DNA replication, recombination and repair; nucleotide transport and metabolism and energy production and conversion. Thirty Mollicute-specific genes were identified, 22 of them previously described as essential genes in Mycoplasma genitalium. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollicutes; Comparative genomics; Synteny; Bidirectional best hit. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200002 Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis BABT Cortez-Herrera,Elizabeth; Sperhacke,Rosa Dea; Becker,Daniela; Kritski,Afrânio; Zaha,Arnaldo; Rossetti,Maria Lucia Rosa. The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase Chain Reaction; Tuberculosis; Internal control; Nucleic acid amplification; Uracyl DNA glycosilase. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006 Restriction-modification systems in Mycoplasma spp Genet. Mol. Biol. Brocchi,Marcelo; Vasconcelos,Ana Tereza Ribeiro de; Zaha,Arnaldo. Restriction and Modification (R-M) systems are present in all Mycoplasma species sequenced so far. The presence of these genes poses barriers to gene transfer and could protect the cell against phage infections. The number and types of R-M genes between different Mycoplasma species are variable, which is characteristic of a polymorphism. The majority of the CDSs code for Type III R-M systems and particularly for methyltransferase enzymes, which suggests that functions other than the protection against the invasion of heterologous DNA may exist. A possible function of these enzymes could be the protection against the invasion of other but similar R-M systems. In Mycoplasma hyopneumoniae strain J, three of the putative methyltransferase genes were clustered... Tipo: Info:eu-repo/semantics/article Palavras-chave: Restriction-modification systems; Mycoplasma spp; Genomes. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200011 Registros recuperados: 4 Primeira ... 1 ... Última

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